Photostimulation and Optogenetics Solutions

Matching Application Requirements

Illumination Power Required	Type of Experiment	Mosaic	Micropoint	Illumination Requirement	Example Applications
	Ablation Studies			Pulsed	Axotomy. Cut microtubules or cytoskeleton. Wound healing assays
	DNA Damage			Pulsed/CW	DNA damage/repair, gene expression, developmental and cancer studies
	Uncaging			Pulsed/CW	Determine function of biomolecules within the cell and/or organism context
	FRAP/FLIP/FRET			CW	Observe protein dynamics, fusion and binding events. Cell compartmentalisation and localization
	Photoswitching/Photoconversion			CW	Study the function of proteins and molecules within complex pathways
	Phenotype Marking			CW/LED	Simultaneous multi-region photoactivation. Simultaneous photoactivation for identification and sorting​
	User Defined Complex Regions of Illumination			CW/LED	Define complex regions of interest: target specific cell structures, groups of cells or tissues
	Optophysiology and Optogenetics			CW/LED	Cell & Neuronal signalling, opsin controlled secondary messengers e.g. Ca2+. Optically controlled protein switching. Signalling cascades. Combine with electrophysiology​

Key:

- Not Suitable

- Suitable for Some Applications

- Partially Suitable

- Recommended

Notes

Pulsed - A pulsed dye laser provides a wide range of wavelengths and high energy at up to 20 pulses per second. While a solid state pulsed laser delivers higher peak power at higher repitition rates.

CW - MicroPoint can be used with CW (continuous wave) lasers for scanned FRAP and other high power density applications. Mosaic can be coupled with Andor's HLE for high power bleaching applications across the visible spectrum.

LED - LED light sources coupled with Mosaic provide illumination powers suitable for many optogenetic probes e.g. ChRh, light-induced dimerization systems and many others.